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Revitalization Program of Floral Garden in Kromeriz
Kudlička, Jan ; Matoušková, Petra (referee) ; Mléčka, Jan (advisor)
This project elaborate the foreground of Kvetna garden in Kromeriz.Everything is solucionated like a fluid connection with an old garden.It isssues from the old concept but however has got a new modern and dynamic form.The main idea is to make a pleasure visit for the people who will arrive and give them more knowings about culture ,history and art.
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Urban design Výstaviště Brno
Kopřivová, Nikola ; Kopáčik, Gabriel (referee) ; Sátora, Josef (advisor)
Diplomová práce se zabývá transformací a novým návrhem funkčního využití území areálu výstaviště v Brně. Areál je vymezen od jihu velkým městským okruhem města Brna a od severu ulicí Hlinky. Cílem návrhu bylo oživit stávající hodnotné území tak, aby se z něho stal fungující městský organismus volně přístupný veřejnosti, který by tvořil symbiózu mezi historickou částí výstaviště a nově navrženou částí.
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School, the Foundation of Life – a Complex of Educational Buildings in Ostrava, Cerna louka
Slavíková, Barbora ; Halfar, Jiří (referee) ; Mikulášek, David (advisor)
The location of Černá louka is situated between the city-centre of Moravská Ostrava and an area of Slezská Ostrava next to the confluence of two rivers – Ostravice and Lučina. The development at the building site of Černá louka is a result from an international competition 2010, which winner was the project Culture Meadow from studio Maxwan. The main idea of the project is to concentrate cultural and educational buildings around a culture meadow. This design has been used as a default situation for the master´s thesis. The complex of schools is situated at the parcel of existing Miniuni-site between cultural cluster Černá louka and the residential quarter Nová Karolina. The volume of the school with its front-spaces reacts to the surrounding structures. In the middle of the complex arises a half-public open space, where are located the internal entrances. Because of the placing of the school next to the city-centre the multifunctional pitch is situated on the roof of the gym. The design of the school respects principles of the age differentiation just as the horizontal integration. That´s why the substance makes up a symbiosis of 5 parts - common space, gym, first and second level, gymnasium. These parts are connected together and at the same time enable to use individual parts separately. The building of kindergarten with its garden is separated from the school because of differential needs of the smallest children.
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Thermal baths Yverdon, five senses in architecture
Krkošková, Kateřina ; Křikavová, Iva (referee) ; Koleček, Ivan (advisor)
Spa building as a symbiotic organism of the park. Principle pouring mass between the trees comes from finding intimacy important for the spa, and the genius loci of the park. The dialogue and the interpenetration of interior and exterior, walking in the park and inside the building, the play of light and vistas are the subject of this work, which produces organic architecture, not as a mere form, but as a result of a thorough analysis of the Site. Park becomes part of the building, creating green bays, thanks to which all the rooms gain the same qualities. Architecture defined by the flow of air and light, scent of spa water. Visitation of the spa as an irreducible complex of impressions that stimulate our senses.
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Diversity, ecology, and biotechnological potential of yeasts associated with bark beetles
Havlíček, Václav ; Kolařík, Miroslav (advisor) ; Novotný, David (referee)
Bark beetles (Coleoptera; Scolytinae) attack woody plants, which is why they are considered pests from a human perspective. They owe their evolutionary success, among other factors, to numerous symbioses with microorganisms, with fungi playing a significant role. The mycobiome of bark beetles is dominated by yeasts, which, nevertheless, are widely overlooked. Intestinal yeasts not only from Ips typographus but also from other species of bark beetles have potential for future biotechnological applications. The aim of this study was to examine their diversity and physiological activities, which will help clarify their ecological role and biotechnological potential. Collections were conducted in the Czech Republic from larvae of the spruce bark beetle Ips typographus and from various species of subcortical insects in Kenya. Yeasts were identified and taxonomically classified based on rDNA sequencing. A total of 133 yeast strains belonging to 37 species were identified, including 9 species that could not be identified and belong to previously undescribed species and in two cases also genera. A total of 133 yeast strains belonging to 37 species were identified, including 9 species that could not be identified and belong to previously undescribed species and in two cases also genera. The most frequently...
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There is something in the air: Diversity of lichen symbionts in the aeroplankton
Veselá, Veronika ; Škaloud, Pavel (advisor) ; Vančurová, Lucie (referee)
Lichens that reproduce primarily sexually rely on the presence of their symbionts in the environment. A new lichen cannot develop without direct contact between the filaments of the germinating spore and a compatible symbiotic alga. However, the results of earlier studies do not provide solid evidence for photobiont presence on lichen-colonized substrates. The objective of this thesis was to assess various environmental types (air, bark, soil, stone, and artificial substrates) as potential sources of symbiotic algae. To achieve in-depth understanding of the diversity of green algae at a long-studied Central Bohemian study-site, Illumina MiSeq metabarcoding of the ITS2 rDNA region was combined with traditional sequencing of lichen algal ITS rDNA and morphological observations. The methods used revealed 283 species-level lineages and 1313 OTUs, many of which belonged to unknown species or higher taxonomic categories. All photobiont species associating solely with sexual lichens at the site could also be found in the vicinity of lichen thalli. As a result, both the substrates and the air, where these symbiotic algae occurred as well, may act as photobiont sources. In contrast, many symbionts that associate only with asexual lichen species, were never found at the site outside of lichen thalli....
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Bacteria in the lichen symbiosis
Moťková, Eliška ; Steinová, Jana (advisor) ; Peksa, Ondřej (referee)
This thesis summarizes the most recent research on bacteria in lichen symbiosis. Lichen symbiosis and the partners participating in it are described in the first part of the thesis. The emphasis is mainly on the different functions that bacteria can have in lichen symbiosis. These functions include, for example, the fixation of atmospheric nitrogen, the production of cobalamin, essential for the growth of some algae, the secretion of antimicrobial substances and the recycling of nutrients contained in old parts of the litter. The main groups of bacteria that occur in the lichen symbiosis are also discussed, along with the factors that influence their occurrence. The most abundant group of bacteria in lichens are the Alphaproteobacteria. Their abundance in lichens is probably influenced by the frequent occurrence of these bacteria in the substrate on which the lichen grows. Other abundant groups of bacteria in lichens are representatives of Firmicutes, actinobacteria or acidobacteria. All important groups of bacteria are acquired by lichens through both vertical transfer and horizontal transfer. Key words: Alphaproteobacteria, nitrogen fixation, antagonistic activity, microbiota, biofilm
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Role of basidiomycete yeasts in the lichen symbiosis
Jerhot, Štěpán ; Steinová, Jana (advisor) ; Černajová, Ivana (referee)
Lichens are, according to the latest definition considered ecosystems, which consist of a fungus, one or more photosynthesizing partners and an undefined number of additional microorganisms. The role of basidiomycete yeasts, which were shown to appear in many lichen species, has been discussed in the latest lichenological scientific papers. This bachelor thesis summarizes the knowledge about the distribution of lichen-associated yeasts and their importance in these symbioses. It also presents some of the methods newly used to study yeasts in lichen symbioses and compares the often inconsistent results based on the methods used in the papers. The topic is not sufficiently covered to draw sufficiently robust conclusions about the role of yeasts in lichen symbiosis. However, the results to date suggest that lichen yeasts do not have any significant specificity of association with mycobiont species, and there has been no support that yeasts directly enhance host fitness. A more detailed depiction of the structures that yeasts form at sites of contact with the host and a description of the mechanisms by which the participating partners communicate with each other could help to definitively determine their role in the lichen symbiosis.
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Co-cultivation of carotenogenic yeasts and cyanobacteria on selected food wastes
Kodajek, Matěj ; Márová, Ivana (referee) ; Szotkowski, Martin (advisor)
Cyanobacteria and yeast are microorganisms, that are cultivated in the biotechnological industry to produce biomass. Biomass contains several organic substances, that are used in the food, pharmaceutical or energy industries. This thesis deals with the joint cultivation (co-cultivation) of cyanobacteria and yeast on food waste. The cyanobacteria Synechococcus nidulans, Synechococcus bigranulatus, Anabaena torulosa and Spirulina were used for co-cultivation with yeast Rhodotorula kratochvilovae and Rhodotorula toruloides. Cultivation media included following food wastes: coffee oil, hemp oil, frying oil, molasses, molasses hydrolyzate, whey, whey hydrolyzate and waste glycerol. In the theoretical part is a comprehensive literature research focused on cyanobacteria, yeasts, and their specific genera, monitored metabolites, co-cultivation, food waste and analytical methods. The experimental part contains a list of used chemicals, devices, aids, strains, and descriptions of procedures for the cultivation of microorganisms, isolation, and analysis of biomass. The aim of the thesis was to do screening cultivations, to find suitable strains of microorganisms for symbiotic co-cultivation and to find a suitable food waste. Following co-cultivation in a semi-operational laboratory bioreactor. Analysis the obtained biomass using gas and liquid chromatography and evaluation the results.
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Complexity of lichen symbiosis
Černajová, Ivana ; Škaloud, Pavel (advisor) ; Palice, Zdeněk (referee) ; Spribille, Toby (referee)
Lichens are currently viewed as complex symbiotic systems. In addition to the main mycobiont and photobiont, a variety of associated fungi, bacteria and algae/cyanobacteria (minor/accessory photobionts) have been recognized. Their diversity has been intensively studied, but is still far from being fully apprehended. Likewise, recognition of the significance of the associated organisms to the whole system is still at the beginning but various crucial roles, from constitutive morphogenetic through physiological to various means of increasing the lichen's fitness, have already been suggested. The present thesis attempts to approach lichens in their full complexity. Focusing on two model systems; the Cladonia-Asterochloris association and ecologically delimited communities of Verrucariaceae; it aims: i) to examine patterns in photobiont choice and their relationship to lichen ecology; ii) to set a framework for in-vitro mycobiont-photobiont compatibility testing; iii) to explore the diversity of selected associated fungi and their possible relationships with the lichen host. We have shown that Verrucariaceae in the intertidal zone associate with largely understudied Ulvophycean photobionts. They mainly belong to Kornmanniaceae, Ulvales, and include a variety of novel lineages, one of which was...
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